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A sample of LF produced in this way, kept frozen at −80°C since 1984, and here designated LF-A/St, was available for use in the current study (Fig. 1).
A truncated LF protein, LF1 254 (also designated LFn), containing the PA binding domain of LF, was shown to follow the N-end rule in cells [19], since addition of destabilizing residues at its N-terminus (e.g., Arg) increased the protein's degradation rate in reticulocyte lysate and cells.
These proteins and LF with the native N-terminal Ala were produced with the Myc tag and factor Xa site, and designated as LF-Z/MyX (Fig. 1, with Z representing the substitution and X denoting presense of a factor Xa cleavage site).
Two isolators were designated as monoassociated; pigs in 1 isolator were orally inoculated with Gram-positive Lactobacillus fermentum (LF) and in the other with a non-pathogenic Gram-negative Escherichia coli (EC).
Figure 1 gives a rough idea of what the thematic mismatches look like (PF = patient focus, LF = locative focus, IF = instrumental focus; the argument at the bottom represents the thematic role of the trigger designated by the corresponding focus/voice morphology): Open image in new window Figure 1 Thematic correspondence between focus morphology and trigger.
We therefore designate the corresponding genes as Gm-collier, Lf-collier and Ek-collier respectively.
LF designed the experiments.
Bonewald LF.
LF Thank you.
Berkman LF.
LF: I agree.
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