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We can suppose that every Chinese citizen would be given a call-list of phone numbers, and at a preset time on implementation day, designated "input" citizens would initiate the process by calling those on their call-list.
Briefly, J774 macrophages (about 4-5×104/well) seeded in 96-well culture plates were infected in triplicate with the transposon mutants, the non-polar deletion mutants, the restriction-deficient strain MFN245, or wild-type F. novicida U112 at the designated input MOI and washed at two hours post-infection (p.i).
One reaction is sufficient to deplete 5 μg of the designated input total RNA.
One aliquot was TCA precipitated and resuspended in 0.2 ml SDS sample buffer and is designated 'Input'.
It runs on a command line with a basic format "java search_dbNSFP -i infile -o outfile," where infile and outfile are the user designated input file name and output file name, respectively.
While in some cases different parameters or reactions could alternatively be modulated by an input, we will regard the designated input as the primary input, since such other modulation either has essentially similar effects or otherwise predictable effects.
Similar(54)
Half of this digested sample was set aside and designated as "input control".
An aliquot of the soluble fraction was set aside and designated as input fraction.
We used the single ranked list option based on human functional annotation, and imported SWISSPROT IDs, which is one of the designated preferred input identifiers for GOrilla analysis.
After centrifugation at 1 x 103 g for 5 min, the supernatant (designated as input) was transferred to a fresh tube and incubated with various monoclonal antibodies as described in figure legends.
Original (raw) cycle threshold values Ct refer to 100 ng of total high quality RNA input (designated 'normalised to RNA input').
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