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For each tissue type, statistical analysis was conducted to determine if there were differences in the masses of tissues designated for extraction via each of the kits.
To avoid including teeth needing treatment only for periodontal, orthodontic, or esthetic reasons, we excluded teeth designated for extraction and veneers or crowns placed on anterior teeth, unless caries was recorded.
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All of the steps were performed using sterile technique in areas designated for RNA extraction and RT-PCR.
The samples were not originally designated for RNA extraction and thus were not consistently handled to maintain RNA integrity.
In all cases, extreme care had to be exercised not to include insoluble curcuminoids in samples designated for butanol extractions, since this would substantially increase the apparent amount of soluble curcuminoids.
Overall, the findings of this study demonstrate that there are practical differences between commercially available RNA extraction kits that should be taken into account when selecting extraction methods to be used for isolating RNA designated for gene expression analysis.
Due to the unascertained risk level of S. aureus strain USA400 (also called MW2), isolation and DNA extraction were performed in biosafety level 3 (BSL3) containment laboratory designated for work with pathogens suspected to be potentially hazardous.
They exclude weapons designated for decommissioning.
Both players were designated for assignment last week.
Meanwhile, infielder Josh Satin was designated for assignment.
Aardsma had been designated for assignment last week.
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CEO of Professional Science Editing for Scientists @ prosciediting.com