A 14 G x 140 mm catheter (Abbocath-T®, Hospira, Slingo, Ireland) was placed on the side designated for blood sample collection (right jugular), while a 16 G x 51 mm catheter (Abbocath-T®, Hospira, Slingo, Ireland) was placed on the side designated for drug administration (left jugular).
The microscope is not designated for biological sample imaging, but this can be allowed for certain cases with specific approval from the lab staff.
Only one entrance was open to the public, while another entrance was designated for bioterrorism sample accession.
The trial was designed such that each pair of plants designated for destructive sampling and the outer edge of every plot was surrounded by guard plants of the same genotype.
In this study, the LAMP reactions were incubated for 60 minutes: a positive result was defined as a positive signal within 60 minutes, while a negative result was designated for samples that did not yield a detectable LAMP product after this time.
After 10 minutes the assay buffer was discarded by vacuum aspiration and 25 µl of assay diluent was added to the wells designated for the samples, while 25 µl of RPMI-1640 with glutamax (GIBCO) was added to the wells designated for standards.
Within each site, the area designated for sampling was blocked at the upstream and downstream ends using seines, which prevented the movement of fish into or out of the sampling reaches during the sampling period.
For comparison, we designated a sample of visits to represent secular trends in screening behavior.
During an LC-MS/MS experiment, peptides designated for tandem mass spectrometry are sampled from a multitude of unknown distributions (Fig. 1).
Fifth, there was a variable number of patient samples in different immunohistochemical analyses, because the second best endomyocardial biopsy sample designated for immunohistochemical stainings was not sufficient for all microscopic slides in every patient (see online supplementary information).
Each sample designated for FCM analysis was divided into two parts for detecting viability (apoptosis and necrosis) and for detecting CD14 and CD11b expression.
