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The designated doses of PAF were administered i.p. immediately after LPS (20 mg/kg), unless otherwise stated.
Beagle dogs (four per group) were injected subcutaneously with designated doses of synthetic glucagon (American Peptide, Sunnyvale, CA), Gcg-XTEN, or placebo.
AGS cells (5×105) or MKN45 cells (1×106) per well in 6-well culture plates were infected with bacteria at designated doses for designated times, washed three times with PBS, and lysed with cell lysis buffer directly on the dish (62.5 mM Tris-HCl (pH 6.8), 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromophenol blue).
Cells were treated with designated doses of Tn for 48 h and incubated with MTT at a final concentration of 0.5 mg/ml for 4 h before the completion of the exposure time at 37 °C.
The treatment groups were orally administered PK (20 mg/kg/day × 3 days), Omez (3 mg/kg/day × 3 days), indomethacin (18 mg/kg × 1 d) as such or along with PK and Omez at the designated doses.
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This unique property, designated dose-duration reciprocity, is a behavior that emerges from the system architecture / system motif.
For LPS-mediated endotoxemia models, mice were injected i.p. with the designated dose of LPS (E.coli O127 B8, Sigma-Aldrich) dissolved in PBS containing 0.25% bovine serum albumin (BSA).
The efficiency of replication of the RSA59 was determined in mice inoculated intracranially at the designated dose.
The groups were designated "multiple doses D0-D8" and "multiple doses D1-D8," respectively.
It is judicious to monitor drug exposure closely when switching preparations since tacrolimus is a designated critical dose drug [ 12].
Technicians were blind for doses (i.e., doses designated by Rx number and color code) to preclude inadvertent bias during all evaluations.
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