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In order to derive a wide range of ME densities, a base diet (designated diet B) was created.
Non-parametric Mann-Whitney U tests were performed for comparing LFD and HFD groups within a time point, and Kruskall Wallis ANOVA with Dunn's post hoc test was used to determine differences between time points within the designated diet.
After three weeks on their designated diet, rats in the fed state were terminally anaesthetised with sodium pentobarbital, hearts and livers were rapidly excised, freeze clamped and stored at −80°C for subsequent analysis.
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Four experimental diets were formulated and designated as diet 1(0%PLM-m-E), diet 2(0%PLM + m-E), diet 3(10%PLM-m-E), and diet 4(10%PLM + m-E).
We designated one diet as the LFHC diet, which was defined as having a relatively high C/F ratio, and the other as the HFLC diet, which had a relatively low C/F ratio.
The prime minister must be designated by Diet resolution, establishing the principle of legislative supremacy over executive government agencies (Article 67).
Greece, Italy, Spain and Morocco have even asked Unesco to designate the diet as an "intangible piece of cultural heritage," a testament to its essential value as well as its potential extinction.
Three replicate groups of fish (23 fish/tank) were assigned to one of three levels of oxygen and one of three diets containing different levels of ascorbic acid (10, 100 and 1000 mg AA equivalent/kg diet) designated as marginal, required and high-dose vitamin in the diets, respectively.
Three parallel RCTs (16, 35, 36) compared a lower-GI diet with diets other than those designated as higher GI (high-fiber diet, traditional diet, very-low-carbohydrate diet) in individuals with type 2 diabetes.
The emperor's major role now consists of such formalities as appointing the prime minister who is first designated by the Diet (Kokkai and appointing the chief justice of the Supreme Court (Saikō Saibansho), convoking sessions of the Diet, promulgating laws and treaties, and awarding state honours all with the advice and approval of the cabinet (naikaku).
A further regime was created for salmon of more than 2.3 kg round weight by the introduction of a lower protein variant of the Advance diet designated ADVLP.
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