In brief, after designated treatment, endothelial cells were scraped off the plates, washed with PBS and dispersed in 5 volumes of ice-cold suspension buffer (100 mM NaCl, 10 mM Tris-Cl, 1 mM EDTA, and 100 µ/mL phenylmethylsulfonyl fluoride).
In brief, we designated the fat body and hemolymph cDNA samples from the bead-challenged pupae as the two "testers" and the cDNA from the samples of the venom treated pupae as the two "drivers".
In brief, after designated treatments, HAECs grown on gelatin-coated coverslips were washed twice by PBS, and fixed by 4% paraformaldehyde solution in PBS for 15 min at room temperature.
"My main feeling is they could have accomplished this without taking what in my experience is the unprecedented step of planting a designated hitter in the briefing room," Peter Maer of CBS Radio, who has covered every president since Jimmy Carter, said after the briefing.
A White House spokesman said the national security adviser, Samuel R. Berger, briefed his designated successor, Condoleezza Rice, on the Middle East in a meeting today.
Her front row seat bears a small plaque with her name, the only seat in the briefing room designated by the name of a person, not a news organization.
The report recommended that each day's brief should be designated a presidential record and eventually examined for declassification.
The 13 appeals court judges study briefs, then meet in New York in panels about 40 days a year in the impressive but crumbling courthouse, designed by Cass Gilbert and designated a landmark.
After all, he had made no secret of the fact that he did not particularly enjoy his brief stint as the designated hitter and had thoroughly missed playing catcher.
After a brief meeting with Syria's designated new leader, Secretary of State Madeleine K. Albright declared today that she found him ready to pursue the peace talks that the Clinton administration has tried to broker between Israel and Syria.
One young girl wearing a Kurt Cobain/Nirvana T-shirt maneuvered the gauntlet to wend her way up the museum steps, recalling fashion's last plunder of rocky youth culture, grunge, and the brief ascendance of what was designated as "heroin chic".
