Age during the fifth (last) larval instar was measured as time since ecdysis: the day of ecdysis to the last larval instar was designated as day 1 of the feeding phase.
To induce an initial phase of demyelination (designated as the 'acute' condition), mice were fed cuprizone continuously for 6 weeks.
To induce prolonged demyelination (designated as the 'chronic' condition), mice were fed cuprizone continuously for 12 weeks.
This period of development is just before the onset of feeding, and is designated as "premetamorphosis" (includes NF stages 45 53).
One room was designated as IAPV+ and the other IAPV−; sampling, feeding, and observations of the bees were done carefully using separate bee suits, gloves and other equipment to minimize contamination.
The dropwise and in-bulk feeding ways of monomers were also designated as method 1 and method 2, respectively.
The diets were designated as CT-1.0, CT-1.5 and CT-2.0, respectively, and fed to lambs on a basal diet of wheat straw to meet requirements for maintenance and growth.
In the present study, we demonstrate that mice fed a high-fat diet from 10to8585 weeks of age and designated Obesity-Prone had significantly higher body and fat pad weights and higher serum leptin levels in comparison with mice fed the same high-fat diet and designated as Obesity-Resistant or Low-Fat.
Animals were fed every 3 days, with the first day designated as Day 0 of RNAi treatment.
A total of 13 isolates, designated as strains GRA-1 to GRA-13, were obtained from 11 ticks (1 fed larva, 5 fed nymph, 1 fed adult female, 1 fed adult male, 1 unfed nymph molted from engorged larva, 2 unfed adult females molted from engorged nymphs) and from 1 pool of eggs and 1 larva derived from the engorged female tick.
Children with a combination of history of prolonged labor, delayed crying, poor feeding, or being admitted to the neonatal unit were designated as having had an adverse perinatal event.
