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The function of any one tRNA molecule is to bind to a designated amino acid and carry it to a ribosome, where the amino acid is added to the growing amino acid chain.
An example of a pair of substances that are enantiomorphs is the two optically active forms of tartaric acid, designated as d-tartaric acid and l-tartaric acid.
We identified 3 zebrafish Rho-GTPase genes, including rhoA (BC075938), rac1 (BC044538) and cdc42 (BC057415), in zebrafish database, cloned and mutated designated amino acids for creations of constitutively-active mutants using QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA) according to the manufacturer's instructions.
Heterozygous flies orc6 35 /Cy, GFP-orc6-wt; orc6 35 /Cy, GFP-orc6-W228A/K229A; orc6 35 /Cy, GFP-orc6-D224A/Y225A contain endogenous orc6 deletion and GFP Orc6 fusion transposons in which designated amino acids were mutated to alanine.
These indels and SNPs were detected and genotyped according to Harel-Beja et al. (2010) [ 18] in genes associated with organic acid metabolism or transport (designated OAMG-organic acid melon genes) that were cloned by two methods: (1) from melon cDNA and gDNA by PCR using degenerate primers based on conserved protein sequences; (2) ICuGI database mining.
In SAR studies a standard nomenclature Pn, … P1, P2, P3 etc. is used to designate amino acid residues of a peptide substrate (Example of P1 group such as branched alkanes and cycloalkanes) [1, 10].
Type I designates amino acid configurations that are very conserved in gene 1 but highly variable in gene 2, or vice versa, implying that these residues have experienced altered functional constraints (i.e., different evolutionary rates) [ 77, 78].
Type II designates amino acid configurations that are very conserved in both genes but whose biochemical properties are very different (e.g., positive versus negative charge), implying that these residues may be responsible for functional specification [ 78, 79].
The Gymnemic acids comprise of several members designated as gymnemic acids I VII, gymnemosides A F, and gymnemasaponins Table 1.
Design, synthesis and characterization of seven linear hydrogen bonded liquid crystal complexes derived from mesogenic p n-decyloxy benzoic acid and p n-decyloxynzoic acids designated as 10OØn (where n varied from ethyl to octyl) are reported.
In addition, such colorless organic acid surface-modified method was also designated as organic acid-coated method [7, 10].
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