Red lines designate rows of water-deficit treated vines and blue lines designate well-watered vines.
The Northumberland Coast Area of Outstanding Natural Beauty (AONB) was designated well over 60 years ago and if you've never been, then you must, because it is very beautiful, wild, remote and a coast worthy of your time and effort to get there.
Master-mix containing the P450 baculosomes reagent, regeneration system, and reaction buffer were added to the designated well of the reaction plate.
Six mm diameter disks were punched and each was placed in a designated well of a 96-well microplate.
A 3.2 mm punch from a dried plasma sample collected on Whatman 903™ filter paper card was placed into the designated well of a 96-well plate.
The cDNA samples were mixed with the RT SYBR Green Fluorescin qPCR Master Mix, then 10 μl of the reaction mixture was placed into designated wells in a 384-well custom-designed PCR array plate.
JERRY ORBACH and SAM WATERSTON, two of the stars of the long-running New York police drama "Law and Order," are among those who have been named living landmarks by the conservancy, which came up with the idea of designating well-known people as living landmarks as a fund-raising gimmick several years ago.
In the reversal experiments, different concentrations of chemotherapeutic drugs (20 µl/well) were added into designated wells after 1 h with or without exposure to potential reversal compounds vardenafil, tadalafil, verapamil, ONO-1078, or FTC (20 µl/well).
100 µl of diluted serum samples, controls, and standards were micropipetted in the designated wells, and then 50 µl of HRP-Conjugate were added into each well and incubated at room temperature for 2 h.
Thereafter, 100 µl of assay diluent was added to the designated wells in a 96-well polystyrene microplate (provided with the kit) coated with polyclonal antibody against IFN-γ, followed immediately by 100 µl of standard, sample or control.
After 3 washes with 200 µl PBS, immunoaffinity-purified FLNA (0.5 µg) from rat brain and A7 cells or MOR (0.1 µg) from rat brain or SK-N-MC cells were added into designated wells and incubation was carried out for 1 hr at 25°C with constant shaking.
