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However, in the cases where the pathways are flanked by long repetitive elements, primer design to amplify across the pathway was impractical.
The sequences obtained from 5' and 3' ends were used for primer design to amplify the complete coding sequence of Psy1 in H. chilense.
The primer pairs were design to amplify a region containing the cleavage site of osa-miMI14, osa-MIR168e and osa-MIR408, respectively.
In each case, Primer3 software was used for primer design to amplify 100 200 bp of sequence based on a 60°C annealing temperature.
However, all of these techniques use specialized NGS protocols including primer design to amplify only MHC class I alleles and amplicon sequencing with long reads (≥150 nucleotides) using Roche/454 GS FLX or Illumina GAIIx.
Incomplete knowledge of a genome's gene repertoire represents a significant bottleneck in biological research as correct gene structures are a prerequisite for computational sequence analysis to determine gene function, for primer design to amplify genes and detect expression, for comparative analysis and for the identification and analysis of regulatory elements and splicing factors.
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Amplifiers are designed to amplify signals.
DNA was amplified by PCR using primer pairs designed to amplify TFPI2 promoter or TET1 promoter.
Also, nested PCR was performed with primers designed to amplify species of the Phylum Firmicutes.
The new treasury analysis is designed to amplify Morrison's opening message.
The primer pair was designed to amplify a region distinguishing the two divergent mitochondrial lineages.
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