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By investigating the relationship between the mRNA secondary structure of translational initiation region (TIR) and gene expression in E. coli, we establish a simple method to design sequences of appropriate TIR (from −35 to +36) that meet a specific expression level as we need.
To demonstrate the entire flow of MRE design, sequences of previously reported human (Homo sapiens) miRNAs were extracted from miRBase [ 16, 17] release 14.
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Development proceeded through a design sequence of cognitive analysis, knowledge representation and workspace design.
Study Design: Sequencing of the p53 tumor suppressor gene has been carried out on 305 ovarian, fallopian tube, and peritoneal cancers.
The algorithm is used for an exact identification of all designing sequences of HP proteins consisting of up to 19 monomers whose conformations are represented by interacting self-avoiding walks on the simple cubic lattice.
By loading such SPIONs with designed sequences of DNA at specific ensemble of the nanopores may upgrade the system to a nano-designed array, the specific details of which is presently under progress.
The designed sequences of oligonucleotide primers for exons 2 and 3 of the KRAS and the operational procedure of direct sequencing were based on those of our previously study [ 18, 32].
We also reveal that a specially designed sequence of pulses can most efficiently enhance the orientation of interacting molecules.
One open question is to what extent the diagnostic process can be automated, replacing the analysts' observations and decisions by a well designed sequence of testing.
Furthermore, pre-incubation of the anti-L1TD1 antibody with the peptide antigen eliminated all bands, suggesting that the anti-L1TD1 antibody is specific to the designed sequence of human L1TD1 gene (Figure 2).
Following the designed sequence of the application, the implemented source code is divided into two sections: GUI and the logic of the program.
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