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Past efforts to design repeat protein-based molecular recognition tools have focused on the creation of templates from the consensus of individual repeats, regardless of their natural context.
We present a novel approach to design repeat proteins of the leucine-rich repeat (LRR) family for the generation of libraries of intracellular binding molecules.
We hope that you will exchange information with Scott Poethig and colleagues on experimental design, repeat a number of crucial experiments under common conditions (which should not take long), and determine if the inconsistencies may in fact be resolvable.
The ability of the repeat A 2-mer to reproducibly induce gene silencing allowed us to use mfold, an RNA structure prediction algorithm [ 25], to design repeat A mutants that would compare the silencing effectiveness when inter- or intra-repeat pairing was enforced.
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A mixed design repeated measures ANOVA was used to test differences between repeated recordings of HR and HRV.
Design: Repeated-measures design.
Design: Repeated measures crossover.
Design: Repeated-measures cohort design.
Design: Repeated-measures engineering testing.
Design: Repeated-measures clinical trial.
Design: Repeated-measures laboratory experiment.
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