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However, a reliable method for the design of the expression process has not been established.
Special consideration has to be given to the choice of expression system, the design of the expression vectors, and the purification strategy to obtain a pure product free from toxins and contamination.
Recombinant lentiviral vectors (rLVs) have emerged as versatile tools for gene delivery applications due to a number of favorable features, such as the possibility to maintain long-term transgene expression, the flexibility in the design of the expression cassettes and recent improvements in their biosafety profile.
Design of the expression vector was as described [24].
The design of the expression construct (pDest-Microarray TT-1) and basic concept of the array platform are shown in Figure 1 and Figure S1.
JM assisted with the design of the expression and regulation experiments and data analysis.
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This emphasizes the influence of the experimental design on the expression profiles, suggesting a high level of inherent variability.
Of primary importance to the design of this expression system was the notion that the strain should produce very little to no endogenous cellulases during the production of the eno-driven Cel7A.
The overall design of this expression experiment is 12 time intervals per cycle, and approximately 25 minutes per time interval.
The overall design of this expression experiment is 12 time intervals per cycle, and 25 minutes per time interval.
Therefore, we transfected COS1 cells with plasmids designed for the expression of FLAG-tagged wild-type or pathogenic TPI enzyme.
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