The design of a VACV construct, GLV-1h68, derived from LIVP wild-type strain by insertion of 3 expression cassettes encoding Renilla luciferase- Aequorea green fluorescent fusion protein (RUC-GFP), β-galactosidase and β-glucoronidase [ 9, 13] lead to a highly attenuated oncolytic virus capable of targeting established human xenografts.
The Language for Learning Research Group, led by Dr. Paola Uccelli, engaged in a systematic review of the literature in order to design a construct of academic language skill that could inform assessment and instruction.
This conceptual idea is demonstrated through a design of a complex constructed wetland system for Huai'an city wastewater plant in Jiangsu province, which sits on the East-route Water Transfer Project of China.
A final control consisted of a construct designed for the overexpression of the Arabidopsis NPR1 protein translationally fused to EGFP (35S: AtNPR1: EGFP).
To evaluate whether PPRE-p-PPAR γ(S112) binding repressed the transcriptional activity of GFAP gene, we designed a construct harboring IR10 and DR1 sequences fused to luciferase reporter, and transfected this construct into cultured rat astrocytes.
The linker between the two molecules is achieved by a design of the construct containing nucleic acids sequence code for the natural hinge region of the human IgG2, which provides flexibility facilitating the ligand-receptor binding [14].
Although preliminary clinical studies have shown the great potential of bone tissue engineering, the constructs employed still lack an integrated approach where clinical needs are met by a careful design of the construct in all its components and growing conditions.
This first paper justifies the need for such repositories and describes the design of a process to construct them from Electronic Health Records (EHR).
The emergence of locked plate technology for periarticular distal femur fracture fixation led to the design of a similar fixation construct for the proximal lateral tibia [17, 18].
Because of this, a construct designed to retarget the Ll.LtrB intron to insert within IS605 needed to be created in three steps.
A new design of a pilot-scale constructed wetland managed with a membraneless microbial fuel cell (CW-MFC) was tested here to assess its potential to generate electricity and remove pollutants under different operating conditions.
