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The sequencing results confirmed that they were 100%% consistent with the initial design (data not shown).
A study on a set of already sequenced strains demonstrated that single replicates where calls of presence/absence were made relative to negative controls showed greater sensitivity and specificity than a reference design (data not shown).
Similar results were seen after adjusting for Jadad score and both Jadad score and study design (data not shown).
The effect of each filter on the resolving power is virtually identical when using oligonucleotides of length 50, 60, 70, or with an isothermal design (data not shown).
Gene expression changes for Cyp genes, IL-6 and SOCS3 were also confirmed by RT-PCR in lung tissues of the same strain of female mice exposed to MTS using an identical exposure system and experimental design (data not shown).
The weighted analysis accounting for sampling design (data not shown) revealed no significant differences between regions and an overall mean duration of interview of 44.3 minutes (95% CI: 41.2, 47.4).
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Restriction enzyme digestion and DNA sequencing assured that all plasmids matched their designs (data not shown).
All designed primers (Table 2) amplified cDNA with a single band at the expected size and of the expected sequence, based on the cassava database sequences from which the primers were designed (data not shown).
This application is very useful when highway design data are not available.
Although, the technique is based on subjective experts' judgment, but is considered very important in designing maintenance plan for existing structures where design data is not available.
Ball-bearing manufacturers supply a wealth of information and design data but not much of it relates to the criteria of particular interest to ultra precision machine designers, namely motion error, static stiffness, damping and heat generation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com