Exact(21)
The lowest concentration consistently detected varied from 0.1 to 10 ng, depending on the locus, indicating the importance of primer design and sequence in the assay.
This paper introduces the development of a formal ontology based on spatiotemporal mereotopology in the context of integrated assembly design and sequence planning.
The first proposed model, following a two-step procedure, determines the cycle length, phase design and sequence, and green split in the first step and optimizes intersection offset in the second step.
Modified Monte Carlo methods are presented in the context of protein design and sequence sampling: Monte Carlo with replica exchange (MCREM) and biased Monte Carlo with replica exchange (BMCREM).
Primer design and sequence information is available elsewhere [7].
Our results show that, despite the differences in probe design and sequence, probe effects average out and may be modeled globally to recover specific transcript concentrations.
Similar(39)
Theory of teacher learning: A situated cognition theory of teacher learning and a cognitive apprenticeship instructional theory guides the design and sequencing of teacher learning experiences.
Specifics of primer design and sequences used in RT-PCR experiments are described in the methods.
PC, VD, ACY and NISC performed the PCR primer design and sequencing.
All details of primer design and sequences are in Fig. 1.
The other three exomes (Mother, Father, and Daughter) were sequenced in January 2013 using the Agilent SureSelect 44 Mb human All-Exon design and sequenced using Illumina HiSeq.
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