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The void penetration length in the SP is considered as a key parameter to design an alignment of Emergency Core Cooling System (ECCS) pump suction pipe in a pool for avoiding the problem of the gas intrusion to the pump.
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The primers and the probe were designed using an alignment of 235 Type 1 (including all subtypes) and Type 2 PRRSV strains.
Degenerate primers were designed from an alignment of all vertebrate LDH-b proteins.
Semi-degenerate primers for conserved regions of TRPV1 and TRPM8 were designed from an alignment of mammalian and chicken sequences of these genes present in GenBank.
ccrA and ccrB of WC28 were obtained using new primers (Table 1) designed from an alignment of known ccrA and ccrB sequences retrieved from GenBank.
Pairs of PCR primers (Table S3) were designed from an alignment of the I. ricinus IxAC coding sequences to specifically amplify each of the family members one at a time, each primer pair generating a product of different size.
In order to design gene-specific primers for 3' RACE, a small fragment (201 base pairs [bp]) in the class I α-2 domain was amplified by PCR using a forward primer described in Radtkey et al. [18] and a degenerate primer (MHCI-R3) designed from an alignment of a range of vertebrate class I sequences (see Table 1 for primer list and sequences; see Figure 1 for primer locations).
This nested assay was designed on an alignment of our HKU1-positive sample sequence (BRA169) and different HKU1 genotype sequences deposited in GenBank.
The left primer was based on the MAGI sequence, and the right primer was designed using an alignment of the MAGI and FST.
Redundant PCR primers (designed from an alignment of HMG domains of a range of Sox proteins) allowed the generation of four novel Acropora PCR products, and for each of these, full-length cDNAs were isolated by library screening.
Primers for the amplification of LEAFY were designed from an alignment of cDNAs of LEAFY of different species of Rosaceae: LFYex1-fwd (5'-CAAGTGGGACCTACGAGGCATGG-3') and LFYex3-rev (5'-TCGGCGTGACAAAGCTGACGAAG-3').
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