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The sample was transferred into a cellulose extraction thimble and stored in a desiccator for 12 hr to complete the desiccation process, then inserted into a Soxhlet apparatus and extracted for 8 hr (seven cycles per hour) with 340 mL solvent mixture n-hexane:dichlormethane (1 1, vol/vol).
Dehydrin isoforms were divided in three groups based on the expression level during the desiccation process in three different tissues (leaves, roots and callus).
Small red1-2 homozygous plants can be successfully rescued by culturing immature seeds, indicating that seed abortion likely results from compromised tolerance to the desiccation process associated with seed maturation.
This paper describes the development of a pseudo-discrete continuum Finite Difference model and its application to investigate the influence of soil properties (including elastic modulus, hydraulic conductivity and soil-water retention) on the desiccation process and eventual crack initiation and propagation behaviour.
Furthermore, the change on water phase in the last stages of the desiccation process is detected by an abrupt decay of the transmitted power for values of the water occupancy close to the 15%.
This is confirmed by the corresponding AFM phase images that show a gradual increase in white zones, with a complete change from dark to white by the end of the desiccation process thirteen hours later (not shown here for the sake of brevity).
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From October, a decline trend was observed until December due to the significant reduction of the vegetation density and activity induced by crops harvest, frequent fires, senescence and desiccation processes during the drought, but also to the soil dryness inducing an evapotranspiration decrease.
After the last rain events, especially during November December, Gp tended to decrease to its lowest value due not only to the starting drought conditions (decrease in soil moisture and increase in VPD), but also to crop harvest, leaf senescence and desiccation processes.
Halomucin is an extremely large protein, homologous to the mammalian mucins, which play an important role in protecting tissues against desiccation processes.
This latter also plays a role in the regulation of desiccation processes during seed maturation [ 14].
Finally, in the late maturation stage, which is also known as the desiccation stage, embryos gradually become metabolically quiescent, with the exception that they synthesize sugars such as sucrose and trehalose to maintain their integrity during the desiccation processes [ 31, 32].
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