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WRA were measured according to the formula: WRA = (desiccated weight − DW /(FW − DW) ×100.
RWC was calculated as follows: RWC = [(desiccated weight – DW /(TW– DW)] × 100.
Water contents (WC) were measured according to the formula: WC = (desiccated weight – DW /(FW – DW) × 100.
For relative water content (WRA) measurements, ten 4-week-old plants with similar size in each line were detached from their roots, immediately weighed (fresh weight, FW) and then left on the laboratory bench (humidity, 45 50%, 20 22°C) until there were no further losses in weight (desiccated weight).
Detached leaves were placed on a bench at room temperature to induce dehydration and weighed at designated time intervals (desiccated weight).
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Samples were weighed at different time intervals (Desiccated Weights, DW).
On this endpoint day, lungs from 5 mice were sampled and weighed, and homogenates thereof were desiccated for dry weight determination.
After a second round of centrifugation, the supernatant was diluted with 3 volumes of EtOH and incubated in a dry ice/EtOH bath for 30 40 min. The resulting suspension was centrifuged at 15000 g for 10 min at 4 °C, the supernatant was removed, and the remaining barium salt was desiccated to a constant weight.
Mature leaves were collected when the branches weighed 90% (onset of leaf wilting), 75% (leaf wilted), or 27% (desiccated) of their initial fresh weight (IFW).
Nematodes (about 3,000 5,000) were desiccated in glass desiccation chambers.
Before analysis, fecal samples were weighted and then desiccated in an oven at 90°C.
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