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A laboratory testing program was carried out using both undisturbed, and remoulded and desiccated samples selected from 20 different locations.
In some cases, desiccated samples were rehydrated with distilled water to soften them before processing.
In the forward run cDNA from desiccated samples was designated as tester, in a reverse run control nematode cDNA was used as tester to investigate both up- and down-regulation of mRNA molecules.
An F-test at a significance level of P < 0.05 was used to compare the ratio of the mean gene expression of desiccated samples with that of the control.
Supernatants were pooled and evaporated to dryness under nitrogen, and desiccated samples were resuspended in 0.25 mL barbital buffer containing 2.5 mg/mL anilino naphthalene sulfonic acid (to disrupt the coupling between thyroid hormones and serum proteins, including lipoproteins), 0.25 mL ethanol, and 1 mL chloroform.
12 Desiccated samples must remain dry to minimize enzymatic activity.
Similar(54)
Compared to the desiccated sample, up-regulated genes after two hours of hydration are primarily related to stress responses.
For functionality test, samples desiccated for 15 minutes were chosen, in view of optimal cell number obtained from viability assay.
Morphological observations were made on algal specimens preserved in 10% seawater or pressed on herbarium sheets, and molecular analysis was conducted on samples desiccated in silica gel.
To better understand the molecular mechanisms of rehydration [ 1, 4, 5, 31, 32, 35, 36] and elucidate the B. argenteum rehydration process at the level of transcriptomics, DEG comparisons between the desiccated and rehydrated samples were performed.
The drying process for our powder is not severe and is not intended to desiccate the sample entirely.
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