A detailed analysis of alternative usage of promoters and polyadenylation sites requires a detailed description of transcript structure and abundance, which is beyond the scope of this study.
cDNA microarrays, corresponding to the analog approach, are used to provide a comprehensive description of transcript level in an organism after perturbation or during development [ 5].
A more detailed description of transcript abundance inside the fungus at 10 dai has previously been described by Tremblay et al. [ 52].
In this report, we present the sequencing, de novo assembly and analysis of the fruit transcriptome and provide a global description of transcript abundance profiles in fruits from shaded and unshaded trees using next-generation sequencing technology in Litchi chinensis.
The Encyclopedia of DNA Elements (ENCODE) Project Consortium [ 42– 44] has facilitated comprehensive discovery and description of genes, transcripts, and transcriptional regulatory regions, as well as DNA-binding proteins that interact with regulatory regions in the genome.
Combined with large cellular microarrays for the assay of cellular gene expression, global descriptions of transcript abundance in specific cells and tissues provides an important component of a total molecular description of the course of viral infection and pathogenesis, which are readily applied to the development of strategies for the treatment and palliation of virus-induced morbidity.
High-level descriptions of transcript expression using hierarchical clustering, and PCA clearly indicated large expression differences by strain and brain region.
The following is a more detailed description of relevant transcripts identified in the cDNA libraries: Of the most abundant transcripts found in the combined analysis of all three libraries were transcripts coding for proteins with similarities to microvilli membrane proteins from A. aegypti and A. gambiae.
These data provide, for the first time, a comprehensive description of the transcripts that are differentially expressed in the embryonic UGE compared to the UGM and reflect the molecular interactions that are likely to occur in the embryonic prostate stem cell niche.
Additionally, only about 2% of genes exhibited a log2 transcript abundance difference of greater than 1.6 (equating to about 3-fold) between these stages (data not shown), which could be explained as a conservative description of the transcripts truly differentially expressed between early- and late-veraison.
