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Simulated metagenomic DNA samples were then subjected to high-throughput sequencing using Sanger sequencing and pyrosequencing technologies (see Methods for a description of the sequencing protocols).
2. There is no description of the sequencing methods.
A detailed description of the sequencing is given in Jakob et al. [ 71].
A more detailed description of the sequencing procedure can be found in supplementary section S1, Supplementary Material online.
The description of the sequencing, alignment, and assignment of estimated quality scores is described in Langley et al. (2012).
A detailed description of the sequencing and bioinformatic analysis of this and following sections can be found in Additional file 2 – Materials and Methods.
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For a detailed description of the sequence analysis, see Coelho et al.51 and Cleary et al.55.55
Instead of a finite number of sequences, such calculations result in a probabilistic description of the sequence ensemble.
A full account of the origin of these errors, with a description of the sequence of events which led to their discovery, has been published in the Empire Surveyors Review (vol. 2, No, 13, July 1934).
"Meaning is what cannot be reached by the immediacy of the image, the description of the sequence of events, the methodologies of expert analysis … Kafka says the writer sees among the ruins 'different (and more) things than others … it is seeing what is really taking place'". Gordimer called it "witness literature".
The description of the sequences in [4, 5] requires some work, so we do not go into the details.
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