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This chapter reveals that the description of tuning database model is incomplete without the description of normalization.
For a detailed description of normalization see [15].
For a more detailed description of normalization and further calculations refer to Methods S1. Results were analyzed using Illumina's BeadStudio software, version 3.1.3.0 and with R, version 2.10.0 [24].
These included comparisons of normalized spike rates at the preferred stimulus orientation for both eyes (Fig. 4A; see MATERIALS AND METHODS for description of normalization procedures) and assessments of orientation selectivity (OSI45, Fig. 4B and OSI90, data not shown) using previously published methods [7], [18], [19].
Detailed description of normalization, time adjustments by expression of lin-42 and sequences of gene-specific oligonucleotides can be found in the Supplementary data.
The histogram in figure 3 displays the relative coverage by CPs of each position along the OR chain (see Methods section 3.4 for a description of normalization of positions between ORs).
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A fully detailed description of the normalization methods was recently published by Schmid et al [50].
A description of the normalization, segmentation, and statistical analyses is available in Supporting information.
A detailed description of data normalization, filtering and averaging of replicates is described in the Methods section.
However, I feel that the description of the normalization procedure was not that easy to follow and would benefit from a small worked example if possible.
A detailed description of the advanced normalization method is beyond this paper.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com