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EMSA probes were generated and labelled and EMSA was performed as previously described with samples analysed on a 1.3% agarose gel (Blackledge et al., 2010).
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Samples were diluted in the same ratio as already described with sample buffer.
4.5 exemplarily demonstrates the different stimuli that have been described with sample measurements.
Nasal passage aspirates, were collected as previously described, with sample from each neonate divided into two sections for detection of viral species by DFA and PCR, respectively.
CD105, TGF β1 and CD105/TGFb1 complexes were determined by immunoassay as described, with control samples taken from 40 healthy volunteers (Li et al, 2000).
The procedure was completed as previously described, with microbiological sampling, careful joint irrigation but without head/liner exchange, and administering the same intravenous empirical antibiotic therapy.
The D fold higher sampling rate allows to model the aliasing effects which possibly occur when considering receive filters with a larger bandwidth as can be described with the sampling rate of the receiver (frac {M M_{text {Tx}}}{T_{mathrm {s}}}).4 Accordingly, the vector (mathbf {n}_{k-N-xi }^{k}) in (3) contains N+ξ+1 vectors each containing MD independent and identically distributed (i.i.d).i.d
Sociodemographic and behavioural characteristics of participants are described, with the sample stratified by sex and sexual behaviour: men who have sex with men (MSM), men who have sex exclusively with women (MSW), and women.
Medium and biomass samples were taken as previously described, with an additional sampling of mycelium from 10 ml broth volume for enzymatic assays.
8-Plex iTRAQ (Applied Biosystems) sample preparation and labeling were performed according to manufacturer's instructions as previously described [23] with samples from Experiment 1 (Table 1).
Morphometric analysis of each optic nerve was performed as described previously with samples from 22 rats [ 20– 20].
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