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For ubiquitination assays, cells cultured and treated as described were collected into glycerol lysis buffer (50 mM Hepes, 250 mM NaCl, 0.5% NP40, 10% glycerol, and 5 mM ethylenediamine tetraacetic acid).
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The monolith herein described was collected from an active acid sulfate soil located near the western shore of the Chesapeake Bay in the Coastal Plain physiographic province in Maryland, US.
The data described here were collected from April through August 2014.
A variety of primary outcome measures, described below, were collected from all dancers before and after intervention.
Data described above were collected and recorded on Teleforms and analyzed using Statistical Package for the Social Sciences, version 16.0 (SPSS; Chicago, IL).
Embryos from the females superovulated and mated as described above were collected at E2.5 and cultured for one day in KSOM media (Millipore) under 5% CO2 at 37°C.
The supernatants of the samples treated as described above, were collected and centrifuged at 25000× g for 10 min at 4°C to eliminate spontaneously formed red cell ghosts.
Blood samples for the studies described below were collected from additional SLE patients and healthy controls at Columbia University Medical Center (New York, NY) and Jacobi Medical Center (Bronx, NY).
The data described below were collected.
Cells described above were collected for cell cycle analysis.
All the below described measures were collected using all the material available after the assessment.
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