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The procedure described under section 2.4 was performed.
Under the normal condition described under Section 3.2.2 in which the BESS converter overloading has not occurred, of course, ΔP m,ref = 0.
LD powder was prepared as described under Section 2. LD extract showed high polyphenol (65 mg equivalent of gallic acid/g of the extract) and flavonoids (80 mg equivalent of rutin/g of the extract) content.
> For each of the six scenarios in Table 3, the outcome, exposures and simulation parameters are described under Section 2 of the Supplementary Material and the values these parameters were set at are available under Section 4 of the Supplementary Material.
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Blackstone believes these factors include but are not limited to those described under the section entitled "Risk Factors" in its Annual Report on Form 10-K for the fiscal year ended December 31 , 2010 as such factors may be updated from time to time in its periodic filings with the SEC, which are accessible on the SEC's website at www.sec.gov.sec.gov
The protease was purified from soluble dialysate by gel filtration chromatography as described under methods section.
Further dilution with the mobile phase was done to obtain the working standard solution to be analyzed as described under the section "General procedure and construction of calibration graphs".
All mass spectra shown in Figures 3, 4, 5, 6 and 7 were generated at a collision energy of 30 eV and 60 eV cone voltage as described under Experimental section.
Details of the protocol are described under Methods section.
cDNA synthesis from total RNA is similar to the procedure described under the section quantitative RT-PCR analysis.
We set different shape parameters and the scale parameter estimates were estimated by fitting the alternative density of z-values (the methodology is described under the section "Fitting the most significant z-values to the alternative density").
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