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Then proceed as described under point 4. Following incubation with appropriate stimuli, pellet neutrophils by centrifugation.
Then proceed as described under point 4. Following challenge of neutrophils (10cells/ml, 400 μl) with stimuli for 1 to 5 min, centrifuge the cells with 400 g for 5 min at 4ºC.
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The participants (n = 10) analyzed the 9 FCS files and reported the results as described under points 16 18 (in silico ICS panel).
As described below under point 6, we have sharply reduced the amount of speculation on the possible role of VPS15 in transmitting signals.
This was used as the guideline during the inception phase, but was presented and amended slightly after discussion with partners at the Inception meeting, principally due to the time limitations (described below under point 2.8).
Bone marrow was extracted from femurs at each time point as described under the section on neonatal femur preparation above.
Arsenic sampling data for each point-of-entry source were used as the outcome variable in our regression model, as described under "Regression Model" below.
General admissions requirements are described under Graduate Education.
These programs are described under Interdisciplinary Programs.
Enrollment under cooperative agreements is described under § 1415.17.
Samples are described under Table S4.
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