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Mutation of tryptophan 170 to alanine (W170A) of HP1β has been described to abolish HP1-PxVxL interaction without affecting HP1 dimerization and H3K9me3 binding [27].
We also generated a Bcl-xL mutant in which tyrosine 101 was replaced with a lysine (Bcl-xLY101K) (Fig. 5A), a mutation that has been described to abolish Bcl-xL's ability to interact with Bax [37].
The other SNP for TIMP-2 in this study (−418G>C), localised in the promoter of the gene, has been described to abolish the Sp1-binding site and therefore may downregulate TIMP-2 gene expression (Hirano et al, 2001).
However, when introducing a D-A mutation which had been described to abolish PB1 specific interactions (Hirano et al., 2004) we were not able to detect any effect on the N-terminal constructs with regard on the nuclear/cytoplasm ratio.
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