Bacteria were grown as described above, suspended in LB (2×105 CFU/ml), mixed with 3 ml of 0.7% soft agar (Sigma) and poured on Petri dishes containing 10 ml LB agar.
Recombinant proteins were prepared as previously described [12], suspended in PBS at a final concentration of 50 µM and added to the culture medium of Suit-2 cells at the final concentration of 50 µg/ml.
Cell walls were prepared from 48 hr 50 ml YPD cultures as described above, suspended in solubilization buffer at 1.5 mg/ml protein then added to plasminogen-conjugated beads at a bead∶protein ratio 1∶2 (v/v) and tumbled for 1 hr at 25°C.
Intact crypts were isolated by Ca2+ chelation as previously described (22), suspended in a storage solution containing (mmol/l): 100 K+ gluconate, 30 KCl, 20 NaCl, 1.25 CaCl2, 1 MgCl2, 10 HEPES, 5 glucose, 5 Na+ pyruvate, 5 Na+ butyrate, supplemented with 1 g/l of bovine serum albumin, buffered to pH 7.4 with 1 mol/l KOH, and kept on ice until required.
It could best be described as "suspend and talk".
The saucily outrageous Ms. Michelson will present "Group Experience," which she describes as "suspended continuous action and calming and soothing movement for seminudes, finger puppets and mental midgets," performed by members of the companies of Merce Cunningham, John Jasperse and others.
(5, 7, 8, 10) and (12) is complete for describing the suspended dust particles effects on the RTI of incompressible rotating fluid.
Albumin samples prepared with acrolein and glucose as described previously were suspended in buffer at a concentration of 0.5 mg/ml in 50 mM HEPES, 100 mM KCL, pH 7.8.
Conidial suspensions of both AC and PC from isolates CLF 29 and CLF 52 were harvested as described above and suspended in 150 µl of 25% alcoholic potassium hydroxide solution and mixed for 2 minutes, followed by incubation at 85°C for 2 hours.
DCs were isolated as described previously and suspended in RPMI medium.
