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Eight categories of best practices are described, supplemented by examples from 5 of 10 ARTAS-II sites.
HepG2.CD81 were generated by lentiviral transduction as described, supplemented with 400 μg/mL Zeocin 5 and polarized by seeding at 1 × 10cells/cm 2 and culturing for 5 days.
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Another respondent described supplementing staff salaries with fundraised dollars because they did not receive enough funding publicly.
PC346C cells were maintained in culture in the complete medium described above, supplemented with 0.1 nM 17-methyltrienolone (R1881; NEN, Boston MA, USA).
These themes are described below supplemented by participants' statements on key themes provided in Table 3.
These themes are described below supplemented by participants' statements on key themes provided in Additional file 1 as supplemental information.
First, the demographic characteristics of clinic attenders were described and supplemented with more detailed medical information on the 1 month subsample.
Stable transfectants were established by culturing transfected cells in the described medium supplemented with 100 μg/ml hygromycin (Invitrogen) for 4 weeks.
H460shBAX/BAK and H460shNT/NT cells were maintained in RPMI as described above, supplemented with 2 μg/ml puromycin and 400 μg/ml G418 (both from Sigma-Aldrich).
The step-by-step method described is supplemented by sample code which can be readily modified for linking any two data sets using standard statistical software.
Cells were cultured in the pancreas media described above supplemented with Y-27632 (10 μM, Sigma-Aldrich) for the first 4 days.
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