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After ubiquitination reactions as described, samples were subjected to SDS-PAGE and a western blot using an anti-Ub antibody was preformed to monitor poly-Ub chain formation.
The density of the described samples SPSed at 1550 °C was 3.21 g/cm3 by helium pycnometry, and it was 3.25 g/cm3 for samples SPSed at 1650 °C.
FMDV primer/probe sets and detection were as previously described; samples with no detectable fluorescence above threshold after 50 cycles were taken to be negative [33].
Simultaneous to these analyses, cell lysates were prepared from the above described samples (1 7) and assayed for the presence of MAD2B and RAN proteins using western blotting.
Microarray for these samples was performed alongside previously described samples.
Although publication dates ranged from 1990 2013, over half of the studies (55.4 %) described samples recruited prior to 1999.
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This study described, sampled and analyzed the soils from each terrace.
At five locations the drillings yielded Roman paleosols that were described, sampled and analysed for soil physical and chemical parameters.
Ten pedons, nine of which are ornithogenic, were described, sampled, and analyzed for physical, chemical and mineralogical properties.
TEM observation was performed by slightly modifying the previously described sample preparation method (Asahi et al. 2014).
3. Test pilot in a described sample.
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