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But the pathway Wernicke had described ran vertically.
The 75-year-old director has described "Ran" as a rendition of Shakespeare's "King Lear" in a 16th-century Japanese setting.
HeLa TAPBPR KO lines were generated using CRISPR as previously described (Ran, et al., 2013).
The second exon of ATG16L1 was targeted in HeLa cells using the px330 plasmid CRISPR system as described (Ran et al., 2013).
Oligos that are listed in a supplementary table were annealed and inserted into the Bbs I sites of pX459 (Addgene, Cambridge, MA, plasmid 48139) by Golden Gate assembly (Engler et al., 2008) as previously described (Ran et al., 2013), using Bpi I (Thermo Fisher Scientific K.K., Kanagawa, Japan) and T4 DNA ligase (New England Biolabs, Ipswich, MA).
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She described running the shelter as an enormous struggle.
One described running past bodies strewn on a street.
He described running for the exit and hearing a man scream: "Come back, save the injured".
She described running away from home at 14 and hitchhiking on a gasoline truck to the Soweto township outside Johannesburg.
She also described running to the Queen after she became convinced that Charles had resumed his romance with Camilla Parker Bowles.
He described running into a man in the movie business who was surrounded by four armed guards.
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