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Immunofluorescent analysis was performed as described previously using the same monoclonal antibody18.
Monocytes were determined using light microscopy and flow cytometry phenotypic analysis of differentiation markers as described previously using CD1441.
Quantitative RT-PCR analysis was performed as described previously using gene-specific primers (Supplementary Data S10 55,56.
LC separations were carried out as described previously using a column packed with porous graphitic carbon.
For microarray experiments, plants were grown on 0.5 MS Difco agar as described previously using 625 and 100 µM phosphate for control and P-deficient conditions, respectively.
The agar diffusion assay was performed as described previously using Mueller Hinton agar [7, 12, 20].
Fingerprints were calculated as described previously using plugins provided in JChem chemistry library.
For comparative analysis, GACCH and GACC were prepared according to the method described previously using 0.2% glutaraldehyde.
The extraction process to recuperate SiO2 is the same as described previously using calcination and acid treatment.
PLGA microspheres were fabricated as described previously, using the water/oil/water double emulsion technique [8],[9].
Nested PCR was performed as described previously using primers specific for the porA gene [19].
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