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Following careful scrutiny of sample identities, all samples that were included in the two previously described pooled node-negative microarray cohorts were removed.
We reaffirmed the prognostic value of the ICS as a continuous variable (computed as described above and Z-transformed) using two previously described pooled expression microarray datasets of untreated node-negative HRneg/Tneg cases as a continuous variable [ 22].
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Cognitive psychology studies have described pooling in illusory conjunction, which is the false perception of an object that is not present yet is composed of "features" from objects that are present (Prinzmetal, 1995; Robertson, 2003; Treisman, 1996; Treisman & Schmidt, 1982).
While there are a variety of reports describing pooled sequencing or multiplexed solution hybridization capture [ 10- 18], our method offers a highly uniform, reproducible workflow for smaller labs that may not have access to high-throughput robotics but still wish to quantify rare variation within specific populations.
To determine whether any of the remaining chromodomain-containing H3K9me3 readers expressed in mESCs are required for SETDB1-mediated silencing, we knocked down Cbx3 (HP1γ) as well as Cdyl2, Cbx1, Cbx2, Cbx5, Cbx7 and Mpp8 in the above-described reporter lines and a previously described pool of mESCs harbouring the silent murine stem cell virus (MSCV) provirus [ 20].
The previously described pools, 1 to 4, mostly include non-sweet melons found growing wild or locally cultivated as exotic vegetables in different parts of the world.
The enrichment in cDNA regulated by the infection was possible by subtraction of cDNA from the 4 described pools against cDNA derived from uninoculated leaves and germinated spores.
Pool et al. (2012) reported a further, mild diversity reduction in Ethiopian highland populations as previously described (Pool et al. 2012), but a lowland sample from far western Ethiopia (EA) showed little diversity reduction.
The authors of the second study described pooling of the infused volume in the mesenteric circulation in patients with advanced cirrhosis (Child Turcotte class C) with no significant increase in central blood volume [ 16].
In 679 Dos Pilas, probably aided by Calakmul, gained an important victory over Tikal, with a hieroglyphic description of the battle describing pools of blood and piles of heads.
As previously described, we pooled the results for "knee pain" and "Heberden nodes" for which both results showed associations with the progression of knee OA.
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