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We smeared an aliquot of the biopsy-homogenates previously described onto Casman agar plates (BBL Microbiology Systems, Cockeysville, MD, USA) for culture of H. pylori.
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The conditions are so deplorable that their skin grows bumps, changes colors, flakes and bleeds....Former prisoner Jason Porter described crowding onto a small table with his fellow prisoners for 30 hours while rivers of human waste 6-inches deep flooded the jail floor after toilets exploded, spraying human feces and urine as high as the ceiling.
Homogenates were then serially diluted and spotted as described above onto LB and LB/Kan25 agar plates.
We mapped the 13 surveillance improvement strategies described earlier onto the process model.
The elements of the intervention that patients described map onto the key features of motivating interventions described by Self-Determination Theory.
PCR templates were generated by cloning mutant animals from among the self-progeny of EG1000/CB4856 F1 hermaphrodites (described above) onto individual seeded plates.
Strains were plated following the complex colony morphology assay protocol described above onto synthetic low-ammonia histidine dextrose (SLAHD) plates to encourage filamentous growth in a nitrogen-poor environment (Gimeno et al. 1992; Liu et al. 1996; Lorenz and Heitman 1997).
LSECs were isolated as previously described [40], seeded onto 6-well flat-bottom plates (Becton Dickinson) at a density of 5×106 cells/well, and kept in culture for 3 days before experiments were performed.
The purification of DAO was done as previously described [ 23], onto a DE52-cellulose and a HiTrap SP Sepharose column.
Combined nose and throat swab specimens in virus transport medium were treated as previously described and injected onto confluent Madin-Darby canine kidney cells (18 ).
Twelve tall fescue seeds, either with seed-vectored rhizobacteria present or with rhizobacteria removed by surface disinfection as previously described, were placed onto plates of both media.
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