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For class switch recombination and differentiation assays BAFF (20 ng/ml R&D Systems) was also included to the previously described medium.
For sucrose treatment, the seedlings were subcultured in the previously described medium or the previously described medium containing additional 90 mM/L sucrose for 7 days.
Cells for the assay were washed twice in the above described medium without IL-6.
Stable transfectants were established by culturing transfected cells in the described medium supplemented with 100 μg/ml hygromycin (Invitrogen) for 4 weeks.
In all the experiments below described medium was changed every 2 days with the sole vehicle (DMSO) or fresh inhibitor MK-04577) added.
Cells (1 × 10 per well at 1-day in vitro (DIV1) were exposed to AMPA plus CTZ (100 μM) in previously described medium for 5 min at 37 °C.
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The comparison of the described medium-sized repeats between different species of the virilis group and other related species may be very helpful in understanding the function and origin of these repeated sequences and their possible role in the evolution of close species of Drosophila.
This paper describes medium optimization for urease production by Aspergillus niger PTCC5011 by one-factor-at-a-time and orthogonal array design methods.
In many works different averaging methods based on the known Hencky-Bolle-type plate assumptions are applied to describe medium thickness functionally graded plates with a microstructure.
The A1B scenario describes medium-to-high greenhouse gas emissions and leads to moderate estimates of global average temperature change of around 3.4°C by year 2100 (IPCC [2007]).
For cell expansion and clonal culture, ALDHhiSSCloVLA4+ cells were plated in a previously described growth medium (NEP medium) containing FGF with or without EGF (13).
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