Kaplan-Meier survival estimates were used to describe lifespan of N. kadleci, and the Log-rank statistic was used to test for significant differences in survival between N. furzeri, N. kadleci and the offspring populations.
Flies were grown under standard culture conditions and aged as described in lifespan assays.
Data were analyzed and plotted as described for lifespan assays.
Experimental worm cultures were set up as described in lifespan assays.
Mated Sir2>S1106-Gal4 fedes fed on medium containing RU486 or ethanol as described for lifespan experiments for 24 h.
As described for lifespan analysis, worms were counted as censored in case of internal hatching, crawling off, and bursting.
A recent paper described extended lifespan of Twi mice treated with a combination of intracerebroventricular, intraparenchymal (Cb) and systemic delivery of AAVrh10 expressing GALC (24).
Worms were cultured in 96‐well plates, as described in lifespan analysis, treated with Mianserin (50 μ m) or water on day 1 of adulthood, and harvested on day 5. Harvested worms (~500 worms) were washed and lysed in a Precellys lysing system.
Median lifespan is the best-suited single parameter to describe a lifespan distribution.
We derive a set of equations to describe the lifespan of an individual cell within the mass.
