Participants described being labeled with a pejorative "Western superiority" that they either personally experienced or observed in others when interacting with their host communities.
Samples were processed as described above and labelled with either APC mouse anti-human P-selectin antibody or isotype control (Becton Dickinson).
Indirect immunofluorescence detection of callose was performed on in vitro germinated pollen tubes, fixed and treated with cellulase and macerozyme as described [ 29], labelled with monoclonal mouse anti-β-1,3 glucan antibodies (1 100, Biosupplies, Parkville, Australia), and visualized with Alexa488-labeled goat anti- mouse antibodies (1 1000, Molecular Probes).
Transverse sections of diaphragm and EDL muscles, prepared as described previously, were labelled with 2 μg/ml WGA-Alexa Fluor 594 conjugate (Molecular Probes, Eugene, OR, USA) in phosphate-buffered saline (PBS), pH 7.4, at room temperature for 1 h according to a modification of the method described by Briguet et al. (2004).
Twenty micrograms of total RNA from a prostate cell line and the reference RNA, isolated as described above, were indirectly labelled with aminoallyl-dUTP (Amersham Biosciences, Piscataway, NJ, USA) by reverse transcription using an oligo dT 15 primer (Roche Diagnostics, Castle Hill, New South Wales, Australia) and Superscript III™ (Invitrogen).
B. cenocepacia genomic DNA was extracted as described [ 56] and labelled with the CyScribe Array CGH Genomic DNA Labeling System (GE Healthcare) at 1 μg per labelling reaction.
Genomic DNA was purified from exponentially growing cells as previously described [ 31] and labelled with fluorophore using the BioPrime® DNA Labelling System (Invitrogen).
Total RNA (500 ng sample-1) was extracted as described herein, then amplified and labelled with a Low RNA Input Linear Amplification kit (Agilent, Waldbronn, Germany).
T cells from OT-II mice (expressing CD45.2) were enriched as described above, and labeled with 4 µM CFSE at 37°C for 15 min. ∼2×106 CFSE-labeled cells were adoptively transferred into congenic CD45.1-expressing BL6 recipients, and allowed to equilibrate for 24 hr.
