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Samples for the conventionalized data set were collected from four wild type mice and four conventionalized TM-IEC C1galt1 −/− mice (colonized as previously described) kept in the Gnotobiotic Mice Facility at the University of Nebraska in Lincoln, Nebraska.
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The conditions the horses were kept in were described as "appalling and completely inappropriate".
The cats were kept in groups under ethologically and hygienically ideal conditions, as described previously (20).
He described being kept in well-guarded hotels and being accompanied by several plainclothes security guards.
Others described being kept in dark and windowless rooms and hearing heavy fighting.
On Tuesday, under questioning by his lawyer, Cristiana Arru, he described having been kept in a small room, with barely enough room to move his arms, with a light on constantly.
The exosome yield was measured with Micro BCA Protein Assay Kit (Pierce) [22] and Vybrand DiI staining (Molecular Probes) as previously described [23] and kept in −80°C until further use.
For immunofluorescence experiments HeLa cells or Raw 264.7 macrophages grown on 12 mm glass slides were inoculated with B. abortus or O. anthropi as described above and kept in the presence of gentamicin for the indicated times.
Arl6ip5 KO mice (Arl6ip5 Δ2/Δ2 ) were constructed as described before and kept in a pathogen-free environment in a standard breeding room.
The collagen gels containing the aortic rings were polymerized in cylindrical agarose wells prepared as previously described (4) and kept in triplicate at 37°C in 60 mm diameter Petri dishes (bacteriological polystyrene, Falcon, Becton Dickinson, Lincoln Park, New Jersey).
The 5% PAA specimens (diameter = 6 mm, thickness = 3 mm) with different concentrations of Bis were prepared using the same protocol as described above and kept in the cell culture medium.
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