Sentence examples for described frozen from inspiring English sources

PBMCs were isolated as described, frozen the same day, thawed the day before the experiment, and cultured overnight.

As previously described, frozen sections were probed with digoxigenin-labeled DR6 antisense probe (5′-TAATACGACTCACTATAGGGGCTGGTGGGTAAGTTGTGGT-3′) and sense RNA probe (5′-ATTTAGGTGACACTATAGAACTCGCGGTACCTTCTCTGAC-3′).

The phonolic compounds were extracted following the method previously described Frozen leaf tissue (2.5 g) was grinded with a mortar and pestle using 15 mL of 50% (v/v) acidified methanol (0.1 M HCl) and the phenolic compounds were extracted for 20 min at room temperature, then centrifuged at 9000g for 30 min.

To describe frozen embryo holders' expectations and perceptions of IVF clinic information, support, and storage fees and their relevance to embryo disposition decision making.

Thin sections of cells prepared using a previously described freeze-substitution method [37] were viewed on an electron microscope H-76000, HitaCo. Co., Tokyo, Japan) at 100 kV.

The resulting time series simulations confirm the model's capability for describing freeze- and thaw-front migration in layered and homogeneous freezing soils.

The delay can be described simply: "Frozen in Canada," said Milos Stehlik, director of Facets.

More Politics News Mr. Bayh, a centrist and the son of a former senator, used the announcement that he would not seek a third term to lambaste a Senate that he described as frozen by partisan politics and incapable of passing even basic legislation.

The brains were cryoprotected in 30% sucrose as described above, frozen in crushed CO2, and cut into sagittal or coronal, 18  μm thick, cryostat sections.

The samples were collected at 10, 20, 30, 60, 120, and 180 min. The medium was removed, and cells were washed with 1× PBS and then scraped into 400 μL of 20 80 phosphoric acid/acetic acid as described above, frozen in dry ice, and kept at −80 °C before thawing for catechol assays.

Consider the binary system (Figure 2) that describes the freezing and melting of the minerals titanite (CaSiTiO5) and anorthite feldspar (CaAl2Si2O8).