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EN made the groEL1ΔC strain, tested its functionality and growth, expressed and purified all proteins described except the AccD5-AccA3 complex and wrote the manuscript.
All experiments were carried out according to the experimental process flow similar to previous described except the pretreatment spent liquor was pH adjusted before added to the solid fraction to conduct enzymatic saccharification [ 32].
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The ΔΔG was calculated as previously described except that the MFE of the miRNA MRE duplex was set to '0' with respect to the ancestral allele.
If a topic is suggested by a participant of the FSGS-CT [focal segmental glomerulosclerosis clinical trial], the writing committee will be formed as just described except that the person making the suggestion may be considered as the lead author.
In all these of cases, a reovirus strain with novel molecular characteristics was described, except for the 1996 case, but the characterization of the isolated strain was not reported.
This was performed with peptides from 2 mg protein in replicate as previously described except that the pH of the mobile phases was 2.7, not 3.0 [3.0.
The cementation process followed the same method as previously described, except that the mantle void was created using one of the formers.
The reagents were the same as previously described except for the following: Potassium bromide (KBr, CAS No. 7758−02−3, ≥99%, FT-IR grade) and graphite standard (GST, CAS No. 7782−42−5, <20 μm, synthetic powder) were from Sigma-Aldrich (St . Louis MO).
The other two are not described, except for the explanation that they are not bound to the peptide because of a crystal contact.
The LTQ-FT was set to perform data acquisition in the positive ion mode as previously described except that the m/z range of 350 1600 was used in the full MS scan [27].
The hf2 phagemid was used to produce phages displaying the scFv after infection as previously described except that the VCS M13 helper phage (Agilent) was used instead of the KM13 helper.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com