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The ascertainment of all outcomes was clearly described (except for wound infection) in six studies.
All instrumental methods including sample preparations were as previously described except for TEM.
All anthracnose resistance genes described, except for co-8, exhibit complete dominance where the dominant allele conditions the resistance reaction.
Most studies that included reaction products were assayed as just described except for the addition of NAD+ or quinols.
The other two are not described, except for the explanation that they are not bound to the peptide because of a crystal contact.
pCEFL-HA- MAPK15, and all its deletion mutants were already described except for 1 300 MAPK15 deletion mutant which was generated by cloning the cDNA, obtained by PCR amplification, in the pCEFL-HA vector.
Similar(41)
SUMO purification was performed as described, except that for gel-filtration analysis a buffer containing 20 mM NaH2PO4/Na2HPO4 pH 6.8, 100 mM KCl, 2 mM DTT was used.
PCR reaction was prepared as described above, excepting for 0.08 μM tailed forward primer, 0.2 μM reverse primer and 0.4 μM fluorescent labelled M13 primer.
Adult C57/BL6 mice were used for all experiments described below, except for the extraction of MSCs from bone marrow, for which 4-week-old C57/BL6 mice were used.
Heat shock was done as previously described [7], except for an incubation at 42°C for 1 hour with recovery at 37°C for 2 hours.
Cycling conditions for both amplifications were the same as described above, except for the annealing temperature, which was 61°C for both reactions.
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