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CM-CQ was synthesized as previously described, dissolved in DMSO and stored at −20 °C.
This reagent has been previously described, dissolved in either acetonitrile [ 39] or ethyl acetate [ 44].
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6*His-AtPSYdTP inclusion bodies were isolated as described [19], dissolved in 10 ml 6 M Gu-HCl, 0.1 M Na2HPO4 (pH 7.15), the protein purified using HIS-Select Spin Columns (Sigma, Taufkirchen, Germany) and dialyzed against water.
[C]HED was synthesized as previously described and dissolved in 50 44 6 0.9% saline/water/8.4% sodium bicarbonate (v/v/v) for injection [ 23].
Total RNA was extracted as described above, dissolved in H2O after LiCl precipitation and purified with the RNeasy Plant Mini kit (QIAGEN) followed by on-column DNase I digestion as per manufacturer's instructions.
Culture DNA was isolated by a cetyltrimethylammonium bromide (CTAB) extraction method as previously described (13 ), dissolved in 10 mM Tris-Cl (pH 8.5), and passed over a Microspin S-200 HR column (Amersham Biosciences UK Ltd ,Chalfont St. Giles, England).
KARPAS-299 human cells were grown as described above, dissolved in sterile PBS to a concentration of 1×10 cells/ml and inoculated subcutaneously (1×10 cells/injection) into the right and left flanks of the mice.
A certain amount of chitaline (as described previously) was dissolved in 20 ml of 2 M ammonia, stirred at room temperature for 1 h and then was filtered.
After end-labeling, samples were precipitated as described above and dissolved in NEB4 buffer supplemented with BSA for Bsp1286I digestion (New England Biolabs).
Insoluble aggregates containing T3SS components were prepared from PA103 supernatants as described above and dissolved in 4% SDS.
Chondramide A (chemical structure Supplementary Figure 1) was isolated as described previously and dissolved in dimethylsulfoxide (DMSO).
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