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After binding of the primary antibody, the washed membranes were incubated with a secondary antibody conjugated to HRP at a 1 5000 dilution for 1 hour at room temperature and washed again as described, developed with a chemiluminescent substrate (Super Signal West Pico, Thermo Scientific), and exposed to X-ray film at multiple exposure times.
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Two subjects described developed hyperglycemia with ketones because of pump site or pod failures in the initial open-loop portion of the study and were rescheduled.
In one incident, he described developing a relationship with an executive in Taiwan who worked at Intersil, a semiconductor company.
Blots were then washed as described above and developed with an ECL kit (Amersham, Arlington Heights, IL, USA).
This particular article describes the theoretical framework developed, with an emphasis on explaining the following major components of the framework: public participation initiatives as a process; policy making processes with a health region; social context as symbolic and political institutions; policy communities; and health of the population as the ultimate outcome of public participation.
We describe STEPS, a stochastic reaction diffusion simulator developed with an emphasis on simulating biochemical signaling pathways accurately and efficiently.
To conclude, the pipeline we have described here was developed with the aim of producing a sorted, enriched subset of true direct regulatory interactions.
Some components of OpenMRS described here were developed with the support of the Rockefeller Foundation.
Nonetheless, Wal-Mart and other retailers have "Code Adam" decals at their stores to reassure customers, and they describe the program, developed with the nonprofit National Center for Missing and Exploited Children, as immensely popular.
In this study, we describe three scenarios developed with Spectrum to explore the expected future changes in the numbers of children living with HIV and the overall number of children needing ART by 2020.
The MEDLINE, EMBASE and HealthStar databases were searched for articles published in English using the keywords and search strategy described in Appendix 1, developed with the assistance of a medical librarian.
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