Cells were transfected as described above, detached from culture plates and resuspended in serum-free Leibovitz's medium containing 0.1% BSA.
It documents three phases in an unnamed author's infatuation with a Bulgarian hustler, and the various settings and transactions involved are described with a detached, carefully styled literary brutalism that feels very of the moment; however, the emotional geography of the story could have come straight from Proust.
HeLa cells seeded and treated as described before, were detached and 0.5% trypan blue dye added in solution.
De Carlos et al. (1996) also described cells that detached from the olfactory epithelium and navigated along the course of olfactory fibers, which they identified as the OMP-ir neurons described by Valverde et al. (1993).
He also describes him as "detached from reality".
The cell number was measured with a cell-counter as described above after detaching the fibroblasts from the surfaces of the samples (Ti6Al4V, TiO2, Cu-TiO2, 2×CuTiO2).
After incubation of MDA-MB231 cancer cells with monocytes, as described above, cells were detached using nonenzymatic detachment solution (Sigma, Saint Quentin Fallavier, France).
Cells were harvested and detached as described above, centrifuged and pellets stored on ice until RNA was extracted using the NucleoSpin RNA II Kit (Macherey & Nagel) as described in provider's instructions.
She also described her as "emotionally detached" and Miles would have tried to talk her out of leaving if he had been made aware of her departure.
Cultured cells were expanded for 3 days as described above before being detached from the culture flasks using 0.05% trypsin−0.2% EDTA (Sigma) and centrifuged at 500 g for 5 min. The cell pellet was fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature for flow cytometric analysis.
