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The cells were examined under a fluorescence microscope at 40 × objective lens magnification. 1 × 10 cells were prepared and treated as described, collected by trpsinization, centrifuged, resuspended in 500 μl PBS and stained with 0.5% trypan blue.
The unstained cells were quantified using a counting chamber. 1 × 10 cells were prepared and treated as described, collected by trpsinization, centrifuged, washed twice with 3 ml PBS, resuspended in 500 μl PBS and stained with 1% Annexin V-FITC/PI, analyzed by FACS caliber (Becton Dickinson). 1 × 10 cells were prepared and treated as described.
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In an article published in Nature in February, Mr. Ginsberg and his five fellow authors described collecting two piles of information -- five years of material from the government tracking how often patients reported flulike symptoms and five years of Google search data -- and then trying to find some overlap.
We therefore focused our study on these previously described collecting sites in the Sierra de Albarracin (September 2008 and May 2009) [ 27] and also identified similar sites at Sierra de Gúdar (May 2009), both province of Teruel, Spain (Table 1).
Previous studies have utilized the moments of the PF to describe collected light intensity near the source [ 28, 32– 32].
This contribution describes collected images, associates time following BCRL onset to the severity and quantity of lymphatic vessel abnormalities in both affected and unaffected limbs, and suggests that cancer related-lymphedema may become a systemic, rather than local, malady.
He describes collecting empty bottles to make Molotov cocktails, the suspension of school for an entire year, the rumor around the neighborhood that some families had fled the country and that one had stashed cyanide tablets "just in case".
David Hunt describes collecting specimens in conditions of cloud, drizzle and overnight temperatures down to -4 °C.
Press coverage of the TfL wi-fi tracking trial has typically described the collected data as anonymized and aggregated.
The monolith herein described was collected from an active acid sulfate soil located near the western shore of the Chesapeake Bay in the Coastal Plain physiographic province in Maryland, US.
We conducted crosses as described, and collected embryos on grape juice agar plates (Genesee) supplemented with live yeast.
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