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DNA fragments were then isolated and analyzed with qPCR using primers from the previously described ChIP experiments.
These results indicate that the described ChIP assay and analysis protocol are suitable for ChIP screening.
As has been previously described, CHIP binds to HSP70 and brings ubiquitin chains to HSP70, leading to subsequent degradation through the ubiquitin/proteasome pathway.
A previously described ChIP protocol was used [ 48], except that the final purification of immunoprecipitated samples was done using a QIAquick® PCR purification kit (Qiagen, Valencia, CA, USA) instead of phenol/chloroform extraction.
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Two recent articles by fellow bloggers for CNN and the Guardian have described chips implanted in the brain to enhance memory and other implants to allow connectivity between brains.
ChIP-chip was performed based on a previously described ChIP-PCR protocol [ 18].
Moreover, the recently described ChIP-exo method can provide nucleotide-level resolution (Rhee and Pugh 2011).
As expected, both replicates of mNET-seq/8WG16 (unph) display strong peaks at the active TSS, consistent with the previously described ChIP-seq/unph profiles.
H-NS and Hha binding profiles were determined using a slight modification of the previously described ChIP-chip and ChAP-chip methods.
This paper builds auger drill models describing chip flow in the auger groove to balance geometric parameters, functional capability, and reliability.
Detailed analysis of genes in these additional categories will be discussed below when we describe ChIP-Seq analysis, which allows differentiation between direct and indirectly regulated members of the CgrA regulon.
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