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Forty freshly decapitated heads from 4 day old OR-control, AβArc-42 flies treated and non-treated with CEppt (as described before) were collected and homogenized in 30 µl PBS/protease inhibitor/1% SDS [16], [32].
PBMCs stimulated for 5 days, as described before, were collected, counted, and seeded in the precoated plates.
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A variety of primary outcome measures, described below, were collected from all dancers before and after intervention.
As described before [32], femurs were collected aseptically from mice and the marrow was flushed out.
S. warneri was also inoculated onto portable equipment such as pulse oximetry devices, electrocardiograph machines, and portable computers as described above and cultures were collected before and after Tru-D disinfection (N = 20).
Protein concentration was determined using a Nanodrop 1000 UV spectrophotometer and subsequently adjusted to 0.62 mg/ml before spectra were collected following the same method as described above.
Organs and blood were collected as described before, and the radiolabelled antibody distribution over time was calculated as the mean %ID/g ± SD for each antibody per time point.
Cells from the bronchoalveolar space were collected as described before [ 5].
Fully expanded leaves of 4-week-old N. benthamiana were infiltrated with agrobacterial suspension as described before [ 50] and leaf samples were collected at 48 hr after infiltration for disease assays and for physiological, biochemical and molecular analyses.
Tryptophan fluorescence spectra were collected before and after titration with different concentrations of CaCl2 as described previously [ 19].
In the experiments described below, generally ten FRAP curves were collected for each set of conditions and averaged before analysis.
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