A one-compartment open model with linear elimination adequately described the data as previously reported [ 3, 5, 19].
Finally, and taking advantage of our study design (4 sites, 2 years) we expect a turnover in species composition within-network but also a constancy in the general patterns describing network topology, as previously reported in other studies [17] [19].
Non-adherent bone marrow cells were cultured as described above and subsequently lysed as previously reported [47].
As previously reported and described above, the decrease or improvement of HbA1c in the intervention group was greater than that in the control group (difference in change: −0.4%, 95% CI: −0.8% to −0.1%, p = 0.02, Table 2).
PCR products for VNTR loci 4, 7, 10, 23, 27, 29, 30, 31, and 36 were assessed using the same PCR reaction as described above and the primers used were as previously reported [ 12].
To study the effects of RB24 on the activation of extraceullular signal-regulated kinases 1,2 (Erk1,2), protein lysates were obtained as described above and Western blot was performed as previously reported (Tari and Lopez-Berestein, 2000).
Identification of protein in the hard corona was performed as previously reported [3] and described below.
Immunofluorescent staining and FA-FISH on filters was used as previously reported [ 18] and described in the supplementary Methods, available at Annals of Oncology online.
Enumeration of CTCs present on filters was done by combining immunofluorescence staining and cytomorphological examination as previously reported [ 18] and described in the supplementary Methods, available at Annals of Oncology online.
